Biochemistry III

COURSE CURRICULUM

1.

Course title

BIOCHEMISTRY III

2.

Code

HM-80B

3.

Study curriculum

Applied chemistry – analytical biochemistry

4.

Organizer of the curriculum (institute, department)

PMF, Institute of Chemistry

5.

Degree (BSc, MSc, PhD)

BSc

6.

Academic year/semester

IV/VIII

7.

ECTS points

6

8.

Lecturer

Dr. Slavica Josifovska

9.

Prerequisites

Biochemistry II

10.

Course objectives (competences):
Students should acquire basic and advanced knowledge of molecular genetics, the molecular basis of inheritance, mutations and their repair, the structure and organization of genes and genomes, as well as the prokaryotic and eukaryotic regulation of gene expression. In addition, it is necessary to become familiar with the basics of recombinant DNA technology, that is, genetic engineering.

11.

Course content:
Regulation of gene expression. Regulation of gene expression in prokaryotes - promoters and the concept of operons. Control of transcription at the operator-repressor level: the lac operon. DNA-binding regulatory proteins; coordinating gene expression; other transcriptional regulation mechanisms. Posttranscriptional regulation of gene expression in eukaryotes; translational and posttranslational regulation. Basics of classical (transmission) genetics. Mendel's first and second laws of inheritance. Molecular aspects of dominance and recessiveness. Deviations from Mendelian laws and other forms of inheritance. Sex-linked inheritance. DNA recombination and gene mapping. Molecular aspects of gene mutations. Biological repair of DNA mutations. Evolutionary significance of mutations. Genomics; prokaryotic genomes; the eukaryotic genome and its expression; repetitive sequences in the eukaryotic genome; structure of protein-coding genes; eukaryotic gene families; human genome. Genetics of bacteria and their viruses. Transcriptomics, Interactomics and Regulatomics. Basic concepts of recombinant DNA technology.

12.

Teaching methods: lectures and experimental work

13.

Total available time

180 hours

14.

Time distribution

3+0+3 hours per week

15.

Teaching methods distribution

15.1.

Teaching - lectures

45 hours

15.2.

Practicals (laboratory, problem solving), seminars, team work

45 hours

16.

Other activities

16.1.

Projects

15 hours

16.2.

Independent work

15 hours

16.3.

Homework

60 hours

17.

Grading methods: Through mandatory 2 colloquiums from the theoretical part or by passing a final exam, a practical exam (with the possibility of passing through two colloquiums or a whole practical exam, as well as with the regularity and activity of lectures.

17.1.

Tests

minimum 40 points, maximum 80 points

17.2.

Seminars/projects (written/oral presentation)

17.3.

Activity

18.

Grading scale (points/mark)

< 50 points

5 (five) (F)

51 to 60 points

6 (six) (E)

61 to 70 points

7 (seven) (D)

71 to 80 points

8 (eight) (C)

81 to 90 points

9 (nine) (B)

91 to 100 points

10 (ten) (A)

19.

Criteria for taking the final exam

The criteria is regular attendance at practical classes (absences of a maximum of 2 exercises). To pass the exam/earn the credits, it is necessary to score a minimum of 56 points. The exam cannot be passed if the minimum points are not obtained from both the practical part and the minimum points from each of the colloquiums for the theoretical part, individually. Points from regularity and activity in lectures are not mandatory, but they take part in the calculation.

20.

Course language

Macedonian

21.

Teaching quality control

Surveys

22.

Literature

22.1.

Compulsory

No.

Author

Title

Publisher

Year

1.

Сашо Панов

Основи на молекуларната биоло­гија и молекуларната генетика

УКИМ

2014

2.

3.

22.2.

Additional

No.

Author

Title

Publisher

Year

1.

Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P.

Molecular Biology of the Cell. 5th edition

Garland Science

2007

2.

Watson JD, Baker TA, Bell SP, Gann A, Levine M, Losick R.

Molecular Biology of the Gene. 6th edition

Benjamin Cummings

2007

3.

4.