Experimental Biochemistry

COURSE CURRICULUM

1.

Course title

EXPERIMENTAL BIOCHEMISTRY

2.

Code

HМ-654

3.

Study curriculum

All chemistry study programs

4.

Organizer of the curriculum (institute, department)

Institute of chemistry, PMF, UKIM

5.

Degree (BSc, MSc, PhD)

BSc

6.

Academic year/semester

III/VI

7.

ECTS points

4

8.

Lecturer

Dr. Natasa Ristovska, Professor

9.

Prerequisites

Organic chemistry II and signature from Biochemistry I

10.

Course objectives (competences):
The aim of this course is students to acquire theoretical knowledge about modern procedures and techniques in biochemistry and molecular biology as scientific disciplines with intensive development. Students should gain practical experience in isolating and purifying biomolecules (proteins, nucleic acids and other smaller molecules), their identification and quantitative determination using classical and some modern chromatographic, electrophoretic and spectroscopic methods.

11.

Course content:
- General laboratory procedures. pH, buffers, biosensors, concentration determination of protein solutions (method according to Biuret and Lowry, methods according to Bradford, BCA and spectrophotometric methods), quantitative determination of nucleic acid solutions (spectrophotometric and other methods), techniques for separating biomolecules from solution by dialysis, ultrafiltration, lyophilization and centrifugal vacuum concentration.
-Purification and identification of biomolecules by chromatography (planar, gas chromatography, column, ion-exchange, gel-exclusive, HPLC, affinity, immunoadsorption and perfusion chromatography).
- Characterization of proteins and nucleic acids by electrophoretic methods (SDS-PAGE, isoelectric focusing, two-dimensional electrophoresis, capillary, immunoelectrophoresis) and practical application of electrophoresis.
- Spectrophotometric analysis of biomolecules (ultraviolet-visible absorption spectrophotometry, fluorescence spectrophotometry, NMR and mass spectrometry).
- Application of radioisotopes in biochemical research. Isotopes in biochemistry, detection and determination of radioactivity, preparation and performance of an experiment, protection.
- Centrifugation in biochemical research. Theoretical foundations of centrifugation, instrumentation and application (preparative techniques, analytical determinations).

12.

Teaching methods: lectures, laboratory classes

13.

Total available time

120 hours

14.

Time distribution

2+0+2 hours/week (lectures 30 hours, laboratory classes 30 hours)

15.

Teaching methods distribution

15.1.

Teaching - lectures

30 hours

15.2.

Practicals (laboratory, problem solving), seminars, team work

30 hours

16.

Other activities

16.1.

Projects

15 hours

16.2.

Independent work

30 hours

16.3.

Homework

30 hours

17.

Grading methods

17.1.

Tests

70 points

17.2.

Seminars/projects (written/oral presentation)

10 points

17.3.

Activity

20 points

18.

Grading scale (points/mark)

< 50 points

5 (five) (F)

51 to 60 points

6 (six) (E)

61 to 70 points

7 (seven) (D)

71 to 80 points

8 (eight) (C)

81 to 90 points

9 (nine) (B)

91 to 100 points

10 (ten) (A)

19.

Criteria for taking the final exam

Finished laboratory practicals and attending lectures

20.

Course language

Macedonian

21.

Teaching quality control

Anonymus surveys for course quality and student suggestions

22.

Literature

22.1.

Compulsory

No.

Author

Title

Publisher

Year

1.

Rodney Boyer

Modern experimental biochemistry, third ed.

Prentice Hall

2000

2.

Наташа Ристовска

Експериментална биохемија (интерна скрипта од предавања за студентите од аналитичка биохемија)

2014

3.

Наташа Ристовска

Практикум по експериментална биохемија (интерна скрипта за студентите од аналитичка биохемија)

2014

22.2.

Additional

No.

Author

Title

Publisher

Year

1.

Robert L. Switzer, Liam F. Garrity

Experimental biochemistry

W.H.Freeman

1999

2.

Nelson, D. L. Cox, M. M Кокс

Lehninger, Principles of Biochemistry

НИП Микена

2011

3.

4.