Chromatographic Methods

COURSE CURRICULUM

1.

Course title

CHROMATOGRAPHIC METHODS

2.

Code

HM-612

3.

Study curriculum

All study programs in chemistry

4.

Organizer of the curriculum (institute, department)

Institute of Chemistry, Faculty of Natural Sciences and Mathematics

5.

Degree (BSc, MSc, PhD)

BSc

6.

Academic year/semester

III/VI

7.

ECTS points

4

8.

Lecturer

Prof. Dr. Marina Stefova

9.

Prerequisites

Analytical chemistry II, Instrumental analytical methods teacher’s signature

10.

Course objectives (competences):
Through the lectures and exercises students should understand and gain knowledge of the basic principles of chromatography as a separation technique, the use of different detection systems (identification and quantification), as well as to gain laboratory experience and the ability to use instruments and process results that will benefit them in their future career in chemical laboratories.

11.

Course content:
–    Introduction: Definition, history, classification of chromatographic methods;
–    Characteristic parameters: Factors affecting retention. Peak widening. Resolution. Quantitative analysis.
–    Thin-layer chromatography (principle, adsorbents, performance, paper chromatography);
–    Column chromatography (principle, adsorbents and applications of: adsorption, distribution, ion exchange, gel, affinity chromatography);
–    Gas chromatography (principle, stationary phases, capillary columns, instrumentation and application).
–    High performance liquid chromatography (modes, theory, instruments, stationary phases, HPLC method development)

12.

Teaching methods: lectures and laboratory classes

13.

Total available time

120

14.

Time distribution

2+0+2 hours weekly

15.

Teaching methods distribution

15.1.

Teaching - lectures

30 hours

15.2.

Practicals (laboratory, problem solving), seminars, team work

30 hours

16.

Other activities

16.1.

Projects

20 hours

16.2.

Independent work

/ hours

16.3.

Homework

40 hours

17.

Grading methods

17.1.

Tests

90 points

17.2.

Seminars/projects (written/oral presentation)

10 points

17.3.

Activity

 / points

18.

Grading scale (points/mark)

< 50 points

5 (five) (F)

51 to 60 points

6 (six) (E)

61 to 70 points

7 (seven) (D)

71 to 80 points

8 (eight) (C)

81 to 90 points

9 (nine) (B)

91 to 100 points

10 (ten) (A)

19.

Criteria for taking the final exam

Attendance at lectures and completed lab exercises

20.

Course language

Macedonian

21.

Teaching quality control

Anonymous surveys, questionnaires

22.

Literature

22.1.

Compulsory

No.

Author

Title

Publisher

Year

1.

A. Braithwaite, F.J. Smith

Chromatographic methods, Fifth Edition

Kluwer Academic Publishers

1999

2.

Zoran Zdravkovski

Fundamentals of Gas Chromatography

PMF, Skopje

2009

3.

D.A. Skoog, D.M. West, F.J. Holler, S.R. Crouch

Analytical Chemistry: An Introduction (in Macedonian)

Prosvetno delo, Skopje

2011

22.2.

Additional

No.

Author

Title

Publisher

Year

1.

D. Harvey

Modern Analytical Chemistry

Prosvetno delo, Skopje

2011

2.

Snyder L., Kirkland J. J., Glaich J. L.,

Practical HPLC Method Development

John Wiley & Sons,

1997

3.

Skoog, Hooler & Nieman,

Principles of Instrumental Analysis

 Saunders College Publishing

1998